6 Questions 29 Answers 0 Followers
Questions related from Mark J. van Raaij
We would like to separate the 30 and 10 kb digestion fragments of an NcoI digestion of a 40 kb linear DNA. They should not only be separated from each other, but also from undigested 40 kb DNA....
10 October 2013 6,744 4 View
We recently obtained the BLR(DE3) strains for some experiments. AFAIK is identical to BL21(DE3) but recombination-deficient. However, we appear to be unable to transform the plasmid pJET with our...
04 April 2013 4,312 4 View
We are considering trying to add additives to bacterial cultures to see if they improve solubility of some proteins we express: L-arginine, glycyl-glycine, sorbitol and betaine. I would be glad to...
02 February 2013 2,673 14 View
Short of sitting with someone and use Coot with them watching and vice-versa, I am wondering whether there is something more efficient like for example a model and map in which certain common...
01 January 2013 1,350 2 View
Since a few months ago, we have been occasionally getting colonies on control plates when transforming with kanamycin-resistance coding plasmids. This happens with different E coli strains. A...
01 January 2013 8,682 11 View
Below things we tried - can anyone add other possibilities? - We are doing fresh transformations with the expression plasmids each time. - We try to induce at the same OD each time and leave the...
10 October 2012 9,437 39 View
