Mark Kan

4 Questions 1 Answers 0 Followers

Questions related from Mark Kan

Endothelial Functional Assays & Conditions?

Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...

06 February 2021 7,427 1 View

Best time-point to determine changes in mRNA via qPCR?

I am trying to determine what best time-point to harvest my cells, HMVEC, after transduction with long non-coding RNA to determine changes in mRNA levels, specifically VEGFR2/KDR. I am capable of...

20 December 2020 4,996 2 View

DNA Transfection Protocols in HMVECs

Hello, I am currently working on a project that requires me to transfect a DNA plasmid (5,600bp) into HVMECs. I want to inquire about available transfection protocols that would yield in high...

01 January 1970 7,817 3 View

'Diabetic' Cells, in-vitro, culturing & confirming characteristics?

I am currently culturing human dermal microvascular endothelial cells obtained from Lonza and want to proceed with experiments comparing diabetic (CC-2930) vs non-diabetic (CC-2543). I am aware...

01 January 1970 4,997 0 View