4 Questions 22 Answers 0 Followers
Questions related from Mark J. van Raaij
We would like to separate the 30 and 10 kb digestion fragments of an NcoI digestion of a 40 kb linear DNA. They should not only be separated from each other, but also from undigested 40 kb DNA....
22 October 2013 5,333 3 View
We recently obtained the BLR(DE3) strains for some experiments. AFAIK is identical to BL21(DE3) but recombination-deficient. However, we appear to be unable to transform the plasmid pJET with our...
13 April 2013 9,190 4 View
Since a few months ago, we have been occasionally getting colonies on control plates when transforming with kanamycin-resistance coding plasmids. This happens with different E coli strains. A...
28 January 2013 3,330 10 View
Below things we tried - can anyone add other possibilities? - We are doing fresh transformations with the expression plasmids each time. - We try to induce at the same OD each time and leave the...
03 October 2012 4,597 38 View
