Maritsa Margaroni

3 Questions 7 Answers 0 Followers

Questions related from Maritsa Margaroni

How can I Wash Leishmania parasites from DC cultures?

I want to do some in vitro infections of dendritic cells with Leishmania parasites. In protocols I have seen , parasites are co-cultured with DCs for 4 h and then those that have not entered the...

01 May 2018 3,365 7 View

Can anyone suggest a protocol for determining adenosine levels in culture supernatants?

I have seen a protocol suggesting using adenosine deaminase to transform adenosine to xanthine in deproteinized media and then measure xanthine, but no details were provided. I have also been...

23 February 2018 2,112 4 View

What sample (serum or plasma) type is better to use in order to detect inflammatory cytokines?

I want to detect inflammatory cytokines or chemokines in mouse model with ELISA. Should I use serum or plasma and if plasma what anticoagulant do you suggest? I find quite controversial...

11 April 2014 9,168 3 View