Has anybody worked with M13K07 helper phage and phage display protocols?

I amplified my stock helper phage by infecting Tg1 at an OD600=0.46 for 90 minutes shaking, spinning down the bacteria, and resuspending the pellet in 2xTY and kandamycin shaking overnight.  I've...

30 October 2015 9,866 10 View