Marina Rocha

3 Questions 1 Answers 0 Followers

Questions related from Marina Rocha

Four-color immunofluorescence: Has anyone had success in staining with four colors on CNS tissue?

I am trying to develop a protocol and would welcome any suggestions you have on serum, etc when working with a variety of different antibody hosts

09 September 2016 174 6 View

Multi color immunfluorescence: does using two different primary antibodies that are derived from the same host (rabbit) interfere with staining?

I currently stain murine tissue with Iba-1 anti-mouse (rabbit host) primary, and then an Alexa Fluor 594 goat anti-rabbit secondary. I want to add a ninjurin-1 stain into this protocol, using an...

09 September 2016 3,040 4 View

Using immunoflourescence techniques, has anyone successfully stained for MHC Class II? If so, with what protocol ?

We use a mouse model

05 May 2016 6,129 1 View