Do you do a selection before harvesting the supernatant from L929? Which media you use to cultive L929? AlphaMEM or DMEM? And to stock?

I´m working with osteoclasts, and once they come from macrophages, I need to add M-CSF in the media. But M-CSF by itself is very expensive and I got some L929, but I am a little bit lost about how...

17 March 2015 7,182 3 View