Marilena D. Papaioannou

3 Questions 4 Answers 0 Followers

Questions related from Marilena D. Papaioannou

Is it normal that transfection with a control LNA (ctA-LNA from exiquon) has an effect on the cells when compared to non-treated cells?

Lately I have been transfecting human embryonic stem cells (hESCs) with an LNA against a specific miRNA (let's call it miR-X). As a control, I use both cells that are transfected with a control...

21 November 2012 2,863 2 View

Cloning the entire length of a predicted 3'UTR

I am having some problems cloning a big 3'UTR for luciferase assays. This particular 3'UTR is 4.6kbs in length, and I cannot clone it after several attempts. Do you think that if I clone a small...

01 May 2012 3,234 12 View

What if the 3'UTR of a predicted target I want to test with Luciferase assays has internal polyA signals?

Hi all, I am trying to clone a big 3'UTR(~3kb) of a gene that is predicted to be a target for my miRNA of interest. When I scan through the 3'UTR sequence I retrieve from Ensemble, I can locate...

23 April 2012 1,544 3 View