Marierose Mina

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Questions related from Marierose Mina

How can I normalise cell count/protein concentration using SDS Coommasie blue stained gel?

Im quite new to cell culture and have had some inaccuracies while harvesting my cells and therefore counting them using a CASY counter. I reconstituted in what I thought to be 50,000 cells/ul,...

17 November 2020 9,966 1 View

No proteins of interest after Co-IP with Dynabeads?

Hello, I am trying to Co-IP my protein complex of interest, and every time i run it on the gel I get nothing but antibody bands. I conjugate my antibody to Dynabeads protein G overnight. I then...

30 October 2020 3,381 7 View

How can I generate a log/log standard curve in R for ELISA analyses?

I carry out ELISA experiments, in which a log/log curve fit is required in order to calculate concentration from O.D. I have used the drc package previously to fit a 4PL model, but this kit...

26 February 2019 8,721 7 View