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My target protein is extracted in PBS, is eluted from column in DAP and neutralised with Tris-HCl, then dialysed against PBS resulting in 4mg/ml. However as I want to do an initial crystal screen...
07 July 2014 8,143 6 View
I am unable to get this information from the manufacturers but would like to know if this matrix requires cyanogen bromide activation prior to use?
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I am using an affinity column with a fetuin-agarose matrix to extract a plant lectin. The crude extract has previously retained some lipid content which has contaminated the column and I would...
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Previously I have used the Bradford assay with a BSA calibration curve to interpolate a concentration for my protein, but now have the actual extinction coefficient (0.1%), I am unsure...
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