Marie Mcconnell

4 Questions 18 Answers 0 Followers

Questions related from Marie Mcconnell

Can anyone help with buffer exchange issues?

My target protein is extracted in PBS, is eluted from column in DAP and neutralised with Tris-HCl, then dialysed against PBS resulting in 4mg/ml. However as I want to do an initial crystal screen...

07 July 2014 8,133 6 View

Does anyone have experience using Fetuin-Agarose from Sigma in an affinity column?

I am unable to get this information from the manufacturers but would like to know if this matrix requires cyanogen bromide activation prior to use?

12 December 2013 5,049 1 View

How can I "clean" my fetuin-agarose affinity media?

I am using an affinity column with a fetuin-agarose matrix to extract a plant lectin. The crude extract has previously retained some lipid content which has contaminated the column and I would...

10 October 2013 4,438 0 View

Having found an extinction coefficient for the protein I am working with, what is the starting point in creating a standard curve?

Previously I have used the Bradford assay with a BSA calibration curve to interpolate a concentration for my protein, but now have the actual extinction coefficient (0.1%), I am unsure...

09 September 2013 311 18 View