Marie Skinner

4 Questions 4 Answers 0 Followers

Questions related from Marie Skinner

How is a mutation suddenly appearing in my PCR product?

I have a PCR product that I've been using as a component that I'm ligating into a vector. Did sequencing it was confirmed to be correct and did a ligation it worked perfect and was sequence...

19 August 2024 7,610 3 View

How is part of my plasmid vanishing?

Hello Everyone! I use Golden Gate technology to build plasmids in my lab. I have a plasmid that I've built that I'm now trying to maxi prep. I've already done a miniprep on this plasmid and sent...

23 April 2024 6,771 8 View

Golden Gate assembly not working?

I'm currently having issues with a golden gate assembly I've been working on for months. The plasmid I'm trying to assemble consists of a backbone- promoter-intron-GOI-Terminator- antibiotic...

14 January 2024 6,117 0 View

Maxi Prep issues

I've been using Golden gate method to create plasmids. I've mini preped and sequenced a plasmid and it was confirmed to be correct. I took this mini prep DNA transformed it and used it to do a...

01 January 1970 5,055 1 View