Mariappan Vignesh

2 Questions 5 Answers 0 Followers

Questions related from Mariappan Vignesh

Why some of the nucleotide sequence is getting deleted from expression vector during transformation?

Hello I am using two expression vectors (pCAGGS and pCDNA) for transforming a fragment of gene (2kbp). For transformation, I am using NEB 10 beta-competent E.coli cells. Issue? Although my...

17 October 2024 9,528 1 View

How to culture elongated shaped HUVEC cells ?

I am using the LONZA bullet kit media (CC-3162) kit for cultured HUVEC. I have revived the cells 5 days back and cultured it in a two T25 flask. But till now I could observe only spherical...

09 December 2019 773 1 View