Mariana Amaral

3 Questions 2 Answers 0 Followers

Questions related from Mariana Amaral

Can anyone recommend buffers mimicking nucleus and cytosol composition for purified protein resuspension?

I want to resuspend a lyophilized protein in buffers that mimick these cellular compartments. Do you know any references about that? I guess I should use a crowding agent but it should be...

10 October 2019 5,430 2 View

What is tRNA level in strain Rosetta (DE3) since it supplies tRNAs for rare codons?

tRNA level in E. coli BL21 strain is ~15% total. I wonder what is tRNA fold increase in strain Rosetta (DE3) since it carries pRARE plasmid.

08 August 2018 3,858 2 View

What's the suggested DNase concentration in the lysis buffer?

My protein (overexpressed in E. coli) is co-purified bound to nucleic acids (High A260/A280 rate). I want to get rid off DNA adding DNAse to the lysis buffer prior to chromatography purification...

11 November 2016 9,806 2 View