Mariana Stein

3 Questions 3 Answers 0 Followers

Questions related from Mariana Stein

How to neutralize the elution buffer (glycine pH 2,8) for MS digestion (in solution) in dynabeads IP?

We are using Dynabeads for IP. According to our protocol, proteins will be eluted with glycine (0.1 M, pH 2.8). We need to proceed tryptic digestion to identify only bound proteins (not...

18 April 2022 3,499 0 View

How to kill a Highlander protease?

We have a purified metalloprotease ( serralisyn from S. marcescens) which stay alive and is able to recover activity when: 1) We heat at 72 degrees for 1 hour 2) Freeze and thaw for 30 cycles 3)...

17 February 2020 5,233 0 View

What is the best way to recover and avoid protease loss during purification?

I'm trying to purify a mettaloprotease, but I have 90% of loss. We use ammomium sulfate precipition, dyalisis (tris 20mM pH8), deae cellulose column (tris 20mM pH 8 and elution with 0.1 NaCl) and...

29 September 2019 8,518 4 View