Mariana Stein

2 Questions 3 Answers 0 Followers

Questions related from Mariana Stein

How to neutralize the elution buffer (glycine pH 2,8) for MS digestion (in solution) in dynabeads IP?

We are using Dynabeads for IP. According to our protocol, proteins will be eluted with glycine (0.1 M, pH 2.8). We need to proceed tryptic digestion to identify only bound proteins (not...

18 April 2022 3,445 0 View

What is the best way to recover and avoid protease loss during purification?

I'm trying to purify a mettaloprotease, but I have 90% of loss. We use ammomium sulfate precipition, dyalisis (tris 20mM pH8), deae cellulose column (tris 20mM pH 8 and elution with 0.1 NaCl) and...

29 September 2019 8,497 4 View