Mariana Murillo-Roos

2 Questions 5 Answers 0 Followers

Questions related from Mariana Murillo-Roos

How to determine enzymatic activity on reconsituted enzyme from lyophilized powder?

I am trying to determine the enzymatic activity of cellulase from Trichoderma reseei ATCC 26921. The product is a lyophilized powder (Sigma C8546) and I am using the NREL method (Filter paper...

19 September 2017 8,194 7 View

How to prepare DNS curve when determining enzyme activity (IU)?

Hello. I am determining the enzyme activity of a cellulase, expressed as umol glucose/mL enzyme/hour. In vials I am adding 1 mL of citrate buffer and 0.5 mL of the diluted enzyme to 50 mg of...

24 April 2017 3,798 3 View