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Questions related from Mariana Franco Fragoso
I generated these RKO Cas9 stable cell line to knock out p53 using different sgRNA (one at a time). To do that, I need to induce the expression of Cas9 with Doxycyclin. I have been using 4ug/ml...
28 August 2019 9,509 3 View
I'm having a lot of trouble trying to do the scratch, because patches of cells come out and then I don't have a perfect line for the assay. I used a p200 tip and washed with PBS after the scratch...
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