Mariam Sameh

3 Questions 5 Answers 0 Followers

Questions related from Mariam Sameh

Why RNA is totally degraded?

I have done RNA extraction from HCT (human colon cell line) using RNeasy mini kit from Qiagen. RNA was totally degraded and no bands could be seen. Could anyone explain why this is happening? I’ve...

19 October 2020 5,396 22 View

How to prepare 37% Formaldehyde from paraformaldehyde?

I want to prepare 37% formaldehyde for denatured formaldehyde agarose gel preparation (for RNA) How can I prepare 37% formaldehyde? And how long this solution would be stable?

12 October 2020 1,275 4 View

What is the concentration of saturated solution of bromophenol blue?

I want to know the concentration of a home-made saturated aqueous solution of Bromophenol blue. How could I know when I add unknown number of grams of the dye?

06 October 2020 3,716 3 View