What is the reason of introduction of SDM 6 nucleotides before the right position in a gene on a plasmid when sequenced?

I am trying to introduce SDM for a gene on a plasmid. After Pcr (with HF Q5 polymerase and primers designed by NEB website the length of one of them 19) , Dpn 1, ligation, transformation and...

28 September 2020 5,599 11 View