Maria Agustina Dominguez-Martin

3 Questions 4 Answers 0 Followers

Questions related from Maria Agustina Dominguez-Martin

Does someone use the Fe3+/ascorbate system?

I'm going to start to work with this system and I have a question, can I prepare the Ascorbate and store it in the fridge for a while or its better to prepare all the time fresh for the assay? Im...

05 May 2013 6,195 2 View

Has the alternative to the Ethidium Bromide the same sensitivity?

I have a question because recently my lab changed Ethidium Bromide to GelRed Nucleic acid stain, from Biotium cat: 41003F. They said that is the same and also less dangerous. But people from...

01 January 2013 3,435 6 View

Did anyone overexpress NtcA from cyanobacteria in E.coli and had problems with the amount after the purification?

I am trying to get NtcA pure and with a good amount from Prochlorococcus. I cloned in a pET15b vector and using the Rosetta strain. And eventually I didn't get enough protein after Ni-NTA...

10 October 2012 6,735 10 View