Why my BV2 cells don't release IL-1B after activation with LPS-ATP?

I activated my BV2 microglia cells with LPS (500 ng/ml) followed by 45 minutes incubation with 5 mM ATP. Although the IL-1B mRNA was 2000-fold increased regarding the untreated cells, I didn't...

13 November 2018 9,355 3 View