Two binding events with stoichiometry of ~0.4/each that turn into a single binding event when I reverse the titration - any thoughts?

I am studying the interaction of Ca2+-calmodulin (CaM) with several calmodulin binding regions (CaMBDs) that have been identified in the protein I work with. One of these shows a strange behaviour...

16 April 2014 1,336 13 View