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Questions related from Maria Letizia Di Martino
Hi! I will perform toeprint assays. I got the tRNA-fMet from Sigma (10U). The only indication from sigma is that 1U will have acceptor activity of 1000 pmoles /A260 unit. In my protocol I should...
15 October 2014 2,501 4 View
I need to recover in vitro transcribed mRNAs from agarose gel, the size of mRNA is more than 1kb. I tried to cut the band and treat it with hot phenol. the protocol works fine but I don't have...
08 February 2014 1,966 9 View