Maria Letizia Di Martino

3 Questions 10 Answers 0 Followers

Questions related from Maria Letizia Di Martino

In which volume should I dissolve tRNA-fMet from Sigma (10U) to obtain a 10uM solution?

Hi! I will perform toeprint assays. I got the tRNA-fMet from Sigma (10U). The only indication from sigma is that 1U will have acceptor activity of 1000 pmoles /A260 unit. In my protocol I should...

15 October 2014 2,536 4 View

Where can I get tRNA-fMet commercially available?

I will perform Toeprint assays on bacterial mRNAs, I need to find the initiator tRNA-fMet.

06 May 2014 8,249 4 View

Does anyone have experience with purification of mRNA after in vitro transcription from agarose gel?

I need to recover in vitro transcribed mRNAs from agarose gel, the size of mRNA is more than 1kb. I tried to cut the band and treat it with hot phenol. the protocol works fine but I don't have...

08 February 2014 2,001 9 View