María Fernanda Rojas Hernández

2 Questions 2 Answers 0 Followers

Questions related from María Fernanda Rojas Hernández

Colony PCR post Gibson Assembly, help?

I did a Gibson assembly reaction and consequently, I did the transformation with my construct to E. coli DH5alpha and I plated the bacteria into 20 µg/mL Kanamycin agar plates. The first time the...

24 January 2024 8,358 3 View

Failed Colony PCR?

Hi everyone, I need some help with my colony PCR procedure. Last Week I did a heat shock transformation on E. coli DH5alpha with pUC57 plasmid. I extracted the DNA from the bacterias by heat,...

06 October 2023 3,461 8 View