Maria Carla Carisì

4 Questions 4 Answers 0 Followers

Questions related from Maria Carla Carisì

In the Base/RNAscope protocol, can I replace the commercial Wash Buffer with SSC or similar?

I use BaseScope 2.5 HD Red quite routinely and I found myself using about 3 liter (1 bottle) of Wash Buffer at every run. Although it is the cheapest component of the kit, it's still quite...

13 November 2019 5,954 3 View

Is there any nucleic acid contamination going from one sample to the next one, when cutting human fresh tissue at the cryostat? RNAse ZAP?

I have 20 fresh frozen brain samples from different patients and I need to cut them using the cryostat (at -20 C), so that 10 slices will go on glass slides for IHC and 15 will go on three tubes...

01 June 2018 3,350 2 View

How do I remove all the HFIP from the amyloid beta preparation?

I am using HFIP to resuspend amyloid beta peptides in order to get amyloid oligomers, following the protocol from prof. Klein. However, HFIP is quite toxic for the cells, so after monomerization...

28 September 2017 9,492 7 View

How do I verify the inhibition of an intracellular enzime by small inorganic molecules in vitro?

I have 100+ small inorganic molecules that have been predicted to inhibit the APT1 enzyme (lysophospholipase 1, citosolic). In order to verify the inhibition in-vivo I would need to do 100+ ELISA...

01 January 1970 7,556 5 View