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I need a good substitute for M9 minimal medium to do expression work of a B. su membrane protein. I have to grow my experimental cultures at 20 oC to slow growth and prevent/ reduce inclusion body...
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M9 medium prepared from M9 salts mixture from sigma. Medium is usually filter sterilized, followed by addition of glucose, CaCl2 and MgSO4. Forms colourless crystalline ppt after 2-3 of storage at...
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