Mari Johnson

5 Questions 7 Answers 0 Followers

Questions related from Mari Johnson

Can you fix cells with Dihydrorhodamine for a respiratory burst assay?

Hi, I'm running an assay measuring phagocyotosis and ROS production in PBMC's. I'm using Dihydrorhodamine 123 (Thermo) to measure ROS. I then stain and fix with PFA afterwards. I've so far read...

10 October 2019 4,204 3 View

How can I 'normalise' my data to a standard curve?

Hi, I'm running an antibody-dependent phagocytoisis assay, where we add serum to fluorescent antigen-coated beads, then donor neutrophils, and subsequently measure the amount of phagocytoisis as...

09 September 2019 6,607 4 View

Can I supplement LB broth with aromatic amino acids?

Hello, I am currently running some experiments that require me to grow up Salmonella Typhi to express the Vi capsule. Currently due to safety restrictions I only have access to using the vaccine...

03 March 2019 9,780 10 View

How to make a plasmid stock from old plate?

So unfortunately I have low amounts of DNA used for my transformation. I still have the plates so was wondering if it's best to just pick a colony from that plate, grow an O/N. make a fresh plate...

01 January 2018 2,659 5 View

Lost DNA after restriction digest?

We used a small amount of our digest (5ul out of 100) and ran on the gel to confirm the digests success, when the rest of the digest sample was quantified with nano drop however, no DNA could be...

07 July 2016 6,313 12 View