Margot Barenstrauch

2 Questions 3 Answers 0 Followers

Questions related from Margot Barenstrauch

Which primers to perform 16S metabarcoding on Iseq100 ?

Hello everyone! We plan to perform 16S metabarcoding on groundwater samples, using the Illumina Iseq100 sequencer. I was wondering which region and primers to use. I would like to perform 2x150...

15 March 2021 844 4 View

Which primers should I use for cDNA amplification (2kb) for further cloning in plasmid?

I want to clone a 2kb cDNA fragment from a fungus gene into plasmid pET28a by homologous recombination but I have troubles to amplify full length cDNA. Up to now we have proceeded like this: 1)...

11 January 2016 7,556 2 View