Has Anyone successfully cloned a 1.5 Kb insert on a 7.5Kb vector with just one sticky end and a blunt end on the other side on both?

We've digested both insert (from purified pcr product) and vector (from maxi prep) with BamH1 ( leaving 1 sticky end) and with HPaI (leaving a blunt end). We have tried the ligation several times...

26 October 2014 2,450 15 View