Marco Túlio Pardini Gontijo

4 Questions 2 Answers 0 Followers

Questions related from Marco Túlio Pardini Gontijo

Bacteriocin annotation: which is the correct name?

I'm predicting bacteriocin gene cluster using Bagel3, Bagel4 and antiSMASH for the same contigs, however, the same cluster and predicted bacteriocin have been given different names (predicted) in...

04 April 2018 3,808 0 View

Is it correct to predict bacteriocins with more than one software?

I'm predicting bacteriocin gene cluster and I'm using 4 different softwares to do it (BAGEL3 and BAGEL4, antiSMASH and MicroScope Mage annotation). I have only seen in the articles of this subject...

04 April 2018 3,174 9 View

How can I do schematic representation of gene clusters?

I'm annotating one cluster and I'd like to make a schematic representation of the cluster (in the same way we found in the publications). I have the whole sequence, the CDS and all informations...

03 March 2018 7,132 2 View

How much IPTG?

I'm going to express acylases in E. coli. How much mM of IPTG should I use for my plasmid? I've seen from 0,1 mM to 1,0 mM in literature, and it has been reported that my protein form "inclusion...

09 September 2017 6,025 5 View