Marco Straus

2 Questions 5 Answers 0 Followers

Questions related from Marco Straus

Can anyone suggest a native protein precipitation or isolation protocol that does not result in DNA contaminations?

I would like to extract proteins from E. coli cells under native conditions to eventually incubate the protein solution together with an in vitro purified protein. However, I need to avoid any DNA...

03 March 2014 7,675 2 View

Malachite Green ATPase Assay - How to deactivate the protein and what is the sensitivity of the assay?

I tried to set up a malachite green assay to measure the ATPase activity of my protein and basically it seems to work but there are a few problems. First, termination of the reaction. I could not...

08 August 2013 5,067 61 View