5 Questions 8 Answers 0 Followers
Questions related from Marco Eigenfeld
Hello everybody, can someone give me a hint how to unmix fluorescence data in GFP and autofluorescence from yeast cells in flow cytometry? Thanks a lot in advance
09 September 2019 6,442 2 View
Hello everyone, I try to purify a chitin binding domain in a native conformation in mono disperse suspension. After purification by use of a polyhistidine tag I dialyze the protein suspension in...
08 August 2019 2,791 7 View
Hi everyone, I want to have an advice for the analysis of flow cytometric analysis of cells by the use of fluorescence intensity and front scatter. I stained yeast cells and want to analyze the...
07 July 2019 2,273 3 View
Hello everybody, i get different results in colony PCR and sequencing. So I'd like to ask for your experience. To my procedure: I made two PCR products of two plasmids by amplification of two...
04 April 2019 4,797 6 View
Hello everybody, I want to analyze yeast cells in flow cytometry (excitation 488 nm) by use of a fluorescence indicator (GFP). I've got the problem, that autofluorescence of yeast cells is higher...
01 January 1970 2,932 8 View
