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Questions related from Marcin Schmidt
Has anyone developed a good protocol for stabilization and subsequent isolation of miRNA from plasma/serum (not whole blood). PAXgene blood lyse cells so it will release intracellular RNA....
09 September 2013 5,176 4 View
I need to differentiate human mature B-cells from non-B-cells (including pre-B-cells), and I would prefer to use a simple qPCR genomic DNA based assay e.g. depending on the amplification of a...
04 April 2013 9,155 9 View
Has anybody tried or use white plates (non-transparent) for Applied Biosystems 7500 Real-Time PCR machine. Are the plates provide better performance? What about background calibration?
03 March 2013 4,370 7 View
I am extracting surface proteins of G(+) bacteria by 5M LiCl extraction (Toba et al., 1995; Turner et al., 1997). I try to concentrate my samples by precipitation with Sigma-Aldrich ProteoPrep...
01 January 2013 4,981 13 View
I am looking for a software that will allow to compare whole bacterial genomes to find conserved sequences. Ideally, the software should be capable for comparing several genomes and return list of...
01 January 2013 7,978 5 View
I did relative comparative qPCR (ddCt) and during data analysis I have selected the most stable gene for endogenous control (from several I have used), also tried multiple endogenous controls....
07 July 2012 708 4 View
