Marcelino Telechea Fernández

2 Questions 3 Answers 0 Followers

Questions related from Marcelino Telechea Fernández

How to overcome to a big differences between biological replicates of rRNA expression in qPCR?

I use b-actin as my housekeeping and I don´t have that problem, and the technical replicates (pippeting) of the 47s rRNA work fine as well, so...I am about to try with the third pair of primers,...

24 May 2017 2,837 2 View

Is there any specific rule to design the dissociation stage (melting curve) at the end of running a qpcr with sybr green?

I mean, I am not able to find if there´s something like a general protocol or the time and the temperatures change according to your conditions? I guess the latter is the correct option but...how??

01 March 2016 8,873 2 View