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Questions related from Marcelino Telechea Fernández
I use b-actin as my housekeeping and I don´t have that problem, and the technical replicates (pippeting) of the 47s rRNA work fine as well, so...I am about to try with the third pair of primers,...
24 May 2017 2,849 2 View
I mean, I am not able to find if there´s something like a general protocol or the time and the temperatures change according to your conditions? I guess the latter is the correct option but...how??
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I am checking several protocols and all of them use leupeptine in their buffers, and I don´t understand how is possible to accurately assess the calpain activity if you are inhibiting it...thanks
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