Marcela Laukova

8 Questions 33 Answers 0 Followers

Questions related from Marcela Laukova

Is there any reliable antibody against corticotropin-releasing hormone (CRH) for Western blot?

Hi guys, does anybody know any CRH antibody which would work for western blots giving a nice and specific signal, especially in brain tissues (PVN, hippocampus)? Any advice is welcome. Thank you.

01 January 2016 9,045 3 View

Troubleshooting the peeled off brain slices from gelatin slides?

Hi guys, I started to work with cresyl violet staining. I am using perfused rat brains with 4% paraformaldehyde, embedded in M-1 matrix from Thermo, 30-40um sections on 2% gelatin slides. I use a...

08 August 2015 9,108 12 View

How do I measure calcium oscillations in primary rat cortical neurons?

Hi guys, I am trying to establish conditions and choose the machine to be able able to measure baseline and stimuli induced calcium oscillations in primary neuronal culture. I tried Cytation from...

05 May 2015 2,779 5 View

Any solution for a problem with adherence of differentiated PC12 cell on poly-d-lysine coated plates?

hi guys, my differentiated PC12 cells do not stick nicely to poly-d-lysine coated well plates. They are easy to wash out after a few washes by pbs or hbss solution. Any idea why and/or what to use...

03 March 2015 8,899 13 View

Can anybody recommend a good and specific p-CREB and CREB antibody for westerns in rat brain tissue samples?

Thanks in advance.

02 February 2014 9,301 5 View

The most appropriate nuclear and cytosolic fraction housekeeping gene?

Could anyone recommend the most appropriate housekeeper for nuclear and cytosolic fraction? I want to isolate both fractions from frozen rat brain tissues and am wondering what is the best and...

08 August 2013 2,175 7 View

Guys, does anybody have a good protocol how to isolate cytosolic and nuclear fractions from tiny frozen brain structures?

I would like to measure glucocorticoid receptor translocation and phosporylated isoforms of this receptor in both fractions of PVN and ventral hippocampus. I would appreciate any advices since I...

07 July 2013 6,389 5 View

Does someone have experience and can recommend a good antibody against phosphorylated forms of glucocorticoid receptor?

I would like to measure levels of different phospho-glucocorticoid receptor forms in rat brain tissues using Western blot. Could someone recommend me any reliable antibody and probably special...

07 July 2013 2,950 29 View