Marcela Laukova

6 Questions 12 Answers 0 Followers

Questions related from Marcela Laukova

How to transfect Tcells with siRNA?

Hi everyone, I am trying to transfect murine CD4+ T cells in vitro, using Lipofectamine RNAi/MAX however, even when trying troubleshoot, the positive FITC labelled control shows just about 3%...

04 June 2020 2,743 1 View

Is there any reliable antibody against corticotropin-releasing hormone (CRH) for Western blot?

Hi guys, does anybody know any CRH antibody which would work for western blots giving a nice and specific signal, especially in brain tissues (PVN, hippocampus)? Any advice is welcome. Thank you.

28 January 2016 419 3 View

Any advice on primary neuronal cell culture from embryonic rat cortex?

Hi guys, I need some advice with primary neuronal cell culture isolated from embryonic cortexes of gestational day 18 old embryos. First, I see a lot of dead cells or small dot-like cells, round...

23 April 2015 161 20 View

Can anybody recommend a good and specific p-CREB and CREB antibody for westerns in rat brain tissue samples?

Thanks in advance.

23 February 2014 7,357 4 View

Any experience with the stability of glucocorticoid receptor protein level in blood lymphocytes after 5-6 hours of storage the blood sample on ice?

I want to isolate lymphocytes from rat blood after decapitation and thereafter measure glucocorticoid receptor protein level by western. However, because of experimental design, rats will be...

27 November 2013 845 0 View

The most appropriate nuclear and cytosolic fraction housekeeping gene?

Could anyone recommend the most appropriate housekeeper for nuclear and cytosolic fraction? I want to isolate both fractions from frozen rat brain tissues and am wondering what is the best and...

06 August 2013 8,589 6 View