Should annealed oligos (20bp) be restriction digested before inserting into cut px330?

I'm using px330 plasmid, cutting it with BbsI, and using annealed oligos that are 20 bp in length. I've used RE with cutsmart buffer. Purified it used a qiagen gel purification column. For the...

10 December 2021 2,077 4 View