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Questions related from Maral Tabrizi
We have some frozen cell-lines in freezing medium (10%DMSO in FBS), which we want to fix using 10% NBF and eventually make FFPE blocks with. Would it be ok to just thaw them rapidly and wash them...
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I have read that I can use RIPA buffer for EV membrane disruption. Does this have to be followed by ultra centrifugation? Does anyone have a protocol for this? Thanks.
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