Mara Esposito

4 Questions 10 Answers 0 Followers

Questions related from Mara Esposito

How to decrease the variation of the fluorescence of G0/G1 populations between samples during cell cycle analysis by Flow Cytometry?

I am performing cell cycle analysis staining the cells with 7-AAD to measure DNA content. I have a big problem, the variation of the fluorescence between samples is quite high. In principle, since...

08 August 2017 1,802 14 View

How do you do the normalisation of X protein to study the effect of a protein Y mutation on X protein level by western blot?

I need to study the effect of the protein Y mutation on the levels of a protein X. So I over-expressed the protein X under 5 conditions: (1) not over-expression of protein Y (2) with...

06 June 2017 7,593 5 View

Is it possible to load 120 ug of proteins in a protein gel?

I need to load 120 ug of proteins in a protein gel. Do I need some precaution for the protein migration and western-blot?

07 July 2016 6,032 5 View

High variability in western blot, why?

I am trying to detect endogenous Aurora B in the whole cell extract but I have high variability during the detection by LiCOR scanner. I use 2 different primary antibodies from BD technology and...

12 December 2015 2,155 7 View