Why I see no RNA bands after fixing and lysate cells, sonicating and extracting RNA with quiazol?

I’ve cultured mouse ESCs and differentiated into neurons. I proceed with PFA 1% fixation, cell lysis using lysis buffer containing 50mM Tris-HCl pH=7, 5mM EDTA, 1% SDS and inhibition cocktail...

09 September 2017 529 3 View