Manuella Silverio

2 Questions 1 Answers 0 Followers

Questions related from Manuella Silverio

Problems with E coli knock out with Datsenko (2000) method with lambda red recombination. What am I doing wrong?

I've been trying to knock out a gene in E coli W. I'm pretty sure I've been following the protocol properly, until the point with the transformation with the linear DNA. The point is, I transform...

15 April 2019 2,924 13 View

Why can't I see any bands after plasmid single digestion, even though the control plasmid (not digested) looks beautiful in the same gel?

I've been struggling with plasmid digestion. I've tried different plasmids, pUC19, pBBR-MCS2, pKD46, with and without cloned genes. For now, I want to single digest them, just to linearise them...

28 February 2019 6,619 3 View