Manuel Jara Espejo

3 Questions 4 Answers 0 Followers

Questions related from Manuel Jara Espejo

How can I convert a nucleotide position from cDNA to genomic position?

I am analyzing data from pseudouridine (https://www.nature.com/articles/nchembio.1836#MOESM157) modifications, where it is reported the position of modified U within cDNA. I need to find the...

01 January 2020 5,043 2 View

Do bigwig file coordinates represent the coordinates of all mapped reads from a RIP-seq experiment?

I am trying to identify enriched regions(peaks) between m6A RNA methylation RIP-seq and input data. For that, the human genome need to be split into non-overlapping 20 nt windows, then I need to...

10 October 2019 3,808 6 View

How can I split a STAR aligner bam file output based on reads strand?

I have bam files (3 IP and 3 input) from a RIP-seq experiment. I need to split each unique mapped reads bam file based on read strand in order to calculate then strand-specific read coverage along...

10 October 2019 7,723 3 View