Mansura Akter

2 Questions 3 Answers 0 Followers

Questions related from Mansura Akter

How to do NMR titraton with a ligand that behaves intermediate exchange at 1/3rd concentration of receptor in solution protein based NMR?

Dear all, I have been trying to measure the least concentration of a nitroxide spin labelled peptide probe to use in paramagnetic relaxation enhancement based NMR experiment. In an PRE experiment...

08 February 2018 3,407 2 View

Hi all, Is there any tips to recover Ni-bound protein that's been get lost with Ni from the sepharose beads leaving it white/inactivated?

I am following an established protocol where during initial Ni-NTA purification the solubilised pr solution (6M GuHCl/0.01M Tris/0.1 M NaH2PO4, pH 8) is incubated with Ni-activated chelating...

10 November 2017 4,334 5 View