How do I co-stain my samples with Picro Sirius Red, DAPI, and Wheat Germ Agglutinin?

I have spheroids (3D microtissues) that are PFA fixed. I want to stain any deposited ECM with Picro Sirius Red (and hopefully confocally/ fluorescently image them), and stain my cells with DAPI...

22 November 2017 3,114 1 View