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Questions related from Maninee Shrivastava
I had gel eluted a PCR product whose concentration according to Nanodrop was 16.5ng/ul. 20ul of this PCR product was treated with 0.5ul of FD Dpn1. Hence, the yield of PCR product in this 20.5ul...
09 April 2023 8,050 3 View
I had done an amplification of pet28a plasmid to introduce some mutations in it using the designed primers. Pcr product was gel eluted, then sequentially was treated with Dpn1 and PNK enzymes....
08 April 2023 6,081 5 View
I want to know about the topics in bioinformatics that are being focused on recently.
16 April 2021 8,424 14 View
In order to prepare a receptor file for docking, we need to remove the ligand associated with the protein; as in the pdb files from Protein Data Bank, coordinates for ligand is also mentioned. So,...
13 April 2020 1,806 10 View
I have following doubts in the analysis part: 1) In Autodock vina, output file is generated. Theoretically, a complex should form after docking which is represented by the output file. Then, on...
30 September 2019 1,350 3 View
