Manibarathi Vaithiyanathan

2 Questions 2 Answers 0 Followers

Questions related from Manibarathi Vaithiyanathan

At what passage number should cultures be stopped for secondary multiple myeloma cell lines, to avoid any genetic modifications?

I have been maintaining secondary lines of OPM2 and MM.1S (both are multiple myeloma cell lines) - currently at P #15. My experiments are quite sensitive since I am looking for specific protein...

18 June 2019 7,835 3 View

I have live & dead cancer cells in ECB. Can I centrifuge/wash excessive stain debris post this stain incubation without getting rid of dead cells?

I am doing a viability test to see the application of Calcein AM and EthD-1 on different platforms. Say, I have sub-populations of live and dead MDA-MB-231's in 2.5 mL of ECB (total cell density:...

23 September 2017 1,669 2 View