Maneesha K Suresh

3 Questions 8 Answers 0 Followers

Questions related from Maneesha K Suresh

What is the best method to refold a recombinant protein purified under denaturing condition which is to be used for immunization?

The His tagged recombinant protein purified using 8M urea precipitated when it was dialysed. The precipitate did not dissolve in PBS. I need to use this protein for immunization. Can anyone...

10 May 2018 4,031 5 View

How should I calculate antibody titer from ELISA result ?

I have used serum sample dilutions from 1:100 to 1:108. I have used a blank without antigen and primary antibody and another blank with antigen but without primary antibody. In both secondary...

15 February 2016 9,756 10 View

Can anyone please explain how to culture T cells from PBMC ?

"T cell - primary culture from peripheral blood." by Raulf-Heimsoth explains it.but I cannot access the paper. The purpose is to study the T cell proliiferation .

14 August 2015 9,022 5 View