Mandy Taisto

4 Questions 6 Answers 0 Followers

Questions related from Mandy Taisto

Does anybody have experience with muscle fiber typing? Any suggestions?

I'm trying to at max label three types of fibers/myosin isoforms with little success in gastroc and sol tissues. So in my KO mouse model I'm trying to assess muscle pathology according to fiber...

05 May 2015 4,216 34 View

Why are my spinal cord sections curled after IHCs?

So I'm working with mouse spinal cords and sometimes the sections are curled/rolled after being sectioned/IHC. It doesn't happen to every batch processed but a good portion do. What would cause...

02 February 2015 2,212 6 View

Any suggestions on a hydrophobic barrier pen that isn't destroyed by triton?

I'm attempting to do an IHC on serial sections of muscle using different antibodies on each section. I'm having troubles keeping the liquid from running into adjacent muscle sections during...

10 October 2013 1,049 1 View

I'm trying to produce large quantities of IgM via hybridomas. Unfortunately we do not have a roller flask set-up. Any suggestions?

Not only do we not have a roller flask apparatus, we are unable to produce these antibodies via ascites method. I need to produce enough antibodies to do rounds of IHC's. Is there anything you can...

07 July 2013 8,815 16 View