Mandana Askarizadeh

3 Questions 4 Answers 0 Followers

Questions related from Mandana Askarizadeh

What is the best qPCR reaction volume you use with SYBR green?

I ahve problem with 20ul final reaction volume. my Positive control works and i have a good amplification curve but my DNA samples do not work! is there any fixed reaction volume that has worked...

01 August 2023 8,338 4 View

Why my PGPR bacteria doesn't grow on TSA medium?

It seems my sample from -80C cannot grow directly on TSA! but it grow on TSA after I use TSB and shaking bacteria first in TSB and transfering in TSA!

01 August 2023 9,760 2 View

Why I have bubbles in my qPCR reaction every time I add SYBR green? How I can prevent making bubbles?

I ma doing a 25ul reaction for my qPCR. I know that making bubbles would destroy my results as it makes DNA polymerase does not work. I use centrifuge after I am done with pipetting but it is not...

11 July 2023 8,860 2 View