Manasi Kamat

4 Questions 5 Answers 0 Followers

Questions related from Manasi Kamat

How do I stimulate macrophages in vitro for cytokine production?

Hello, I am trying to study the cytokine profile of PDL1+ TAMs and will be isolating them from human tumor tissue. I am confused as to directly doing intracellular staining for my cytokines of...

08 August 2017 6,160 4 View

Has anyone used enzymatic digestion to isolate myeloid cells from human breast tumors? If yes, which enzymes and could you please share the details?

I need to isolated myeloid lineage cells from human breast cancer tumors. Downstream applications would be tissue culture, flow cytometry and functional assays. In the lab we use enzymes Liberase...

10 October 2016 6,147 3 View

I need to label plated macrophages with CFSE? Has anyone does this before?

I have always labeled cells with CFSE after detaching them from the culture plate. However I need to label plated macrophages this time. What I usually do is use the required concentration of CFSE...

11 November 2015 1,588 4 View

How do I differentiate monocytes into a phenotype similar to Tumor-associated macrophages (for breast cancer)?

Would a good approach be to use supernatants from various breast cancer cell lines and differentiate these CD14+ monocytes from healthy donor blood into macrophages in their presence for 10-14...

01 January 1970 1,995 2 View