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Hello, I‘m working with ready made primers for 3 genes (one of them is GAPDH- the housekeeping gene) in taqman gene expession assay. The GAPDH primer has VIC dye while the other two primers have...
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Hello, I have formalin-fixed paraffin-embedded samples of breast cancer and I want to extract RNA from them. I tried several times with the RNeasy FFPE Qiagen extraction kit, but I usually get low...
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Hello, I have 2 questions if anyone can help: 1) I have 2 groups (treatment and control), and the outcome is categorical (8 categories). I used chi2 square test and found significant difference...
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Hello, I have an independent binary variable & a dependent ordinal variable. I want to test the correlation between them & found that rank-biserial correlation coefficient will be the most...
01 January 1970 4,668 9 View
Hello I was wondering where to add the asterisk on a bar graph representing proportions of a three-levels ordinal variable in 2 groups. The Mann-whiteny U test that I use to detect if there is a...
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Hello, If anyone can help, I don't understand what is written in the upper limit for confidence interval for IV.2 "+inf", and why does it appear here, and how to interpret it. Also what does...
01 January 1970 3,803 4 View
Hello, I have 2 groups of patients (treatment and control). Each individual in both groups took 16 cycles of treatment (let's say: treatment A for treatment group & B for control group). After...
01 January 1970 9,863 4 View